How to collect blood by venipuncture

1. Select the vein site:
   For most venipuncture in adults, veins located in the arm are used. The median cubital vein is the one used for the patient

• Factors in site selection
  (i) healed burn areas should ne avoided
  (ii) Hematoma

1. Applying the tourniquet
   

• A tourniquet makes the vein more prominent. But tourniquet should never be left on arm for more than two minutes.

1. Performing venipuncture

   • The patient's arm is gripped tightly and thumb of another hand is used to draw skin taut.

   • The venipuncture by placing needle at 30° to 40° angle)

   • Draw the blood very carefully

   • After blood has been drawn, the patient should release the fist and the tourniquet is also released

   • Apply cotton without spirit on punctured area.

   *After removing the needle the collected blood dispense in the vials

   • red vial: for biochemistry and microbiological test
   • violet (EDTA ) for hematological test
   • gray (fluoride oxalate) for blood glucose
     In anticoagulated vials blood should be mix properly

   * After venipuncture the needle should be removed from the syringe and disposed by using needle destroyer
   * Dispose used cotton balls, in yellow box (box for infected material)
   

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