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Ronaldinho, 37, was part of the triumphant Brazil 2002 World Cup squad, won the Champions League in 2006 with Barcelona and won the Balon d'Or in 2005.
His brother and agent Roberto Assis confirmed the retirement on Tuesday and announced a series of tribute events.
"He has stopped, it is ended," Assis said.
"We will do various events in Brazil, Europe and Asia and, of course, we are arranging something with the Brazilian team as well..