Alexander Fleming serendipitously discovered the antibiotic effect of the fungus Penicillium rubens in 1928 when working with staphylococci cultures (Fleming 1929). One such culture was contaminated with a fungus, and the colonies of staphylococci around the mold were destroyed, whereas other colonies farther away were unaffected. He grew the fungus in pure culture, established that it also killed other disease-causing bacteria, and named the unknown active ingredient penicillin.
Only 12 years later the pure substance was isolated and characterized and its chemical structure determined. The success of penicillin and its derivatives triggered a search for additional antibiotics produced by other fungi and led, for example, to the discovery of cephalosporins which have the same mechanism of action but are less prone to hydrolysis by bacterial β-lactamases.
the structure of penicillin:
penicillin synthesis:
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