It was said that multiplex PCR is a type of PCR technique which allows an amplification of many target sequences concurrently in the same reaction mixture.A single reaction mixture includes sets of primer pairs for different DNA targets. It reduces the consumption of PCR reagents, and, at the same time, imposes restrictions on used primers.Technology is giving us a lot of opportunity, wanna know what are those? Then, read this sponsored post; https://cutt.ly/PotentialOfTestKitInTheMarket
To work properly within one reaction, sets of primers must be optimized. They have to have similar annealing temperatures and produce amplicons of different sizes to form distinct gel electrophoresis bands for the followed PCR analysis. Check disclaimer on profile and landing page.