RT-PCR (Reverse Transcriptase PCR)

Accordingly, RT-PCR, also known as Reverse Transcriptase PCR, is a variation of the polymerase chain reaction that typically measures RNA expression levels. In RT-PCR, complementary DNA (cDNA) is said to be made by reverse transcribing of the RNA templates with the enzyme reverse transciptase. Find out more information in this sponsored post and see if it is right for you. https://cutt.ly/PotentialOfTestKitInTheMarket

This technique is used to qualitatively study gene expression, and could be combined with real time PCR (qPCR) to quantify RNA levels. RT-PCR is used in research laboratories to study gene expression, for example in experiments to distinguish exons from introns, and might be used clinically to diagnose genetic diseases and monitor drug therapy. RT-PCR potentially requires an RNA template, enzyme, nucleotides, buffers and thermocyclers to potentially produce RT-PCR products. Check disclaimer on profile and landing page.