Um, writing something on your own? Lets look at that...
Your text-
Mammalian Cell Expression Vectors:
Mammalian expression vectors are useful for the production of specific and authentic recombinant proteins (for use as therapeutic agents). In addition, they are also helpful for studying the function and regulation of mammalian genes. In general, the mammalian expression vectors are quite comparable to other eukaryotic expression vectors. . However, large-scale production of recombinant proteins with engineered mammalian cells is costly.
A diagrammatic representation of mammalian vector is shown in the figure below. It contains a eukaryotic origin of replication from an animal virus such as Simian virus 40 (SV40) and a prokaryotic origin of replication (from E. coli). The mammalian vector has a multiple cloning site and a selectable marker gene. Both of them are under the control of eukaryotic promoter and polyadenylation sequences.
These sequences are obtained from either animal viruses (SV40, herpes simplex virus) or mammalian genes (growth hormone, metallothionein). The promoter sequences facilitate the transcription of cloned genes (at the multiple cloning site) and the selectable marker genes. On the other hand, the polyadenylation sequences terminate the transcription. Ampicillin resistant marker gene can be used for selecting the transformed E. coli cells.
Source text-
Mammalian Cell Expression Vectors:
Mammalian expression vectors are useful for the production of specific and authentic recombinant proteins (for use as therapeutic agents). In addition, they are also helpful for studying the function and regulation of mammalian genes. In general, the mammalian expression vectors are quite comparable to other eukaryotic expression vectors. . However, large-scale production of recombinant proteins with engineered mammalian cells is costly.
A diagrammatic representation of mammalian vector is shown in Fig. 11.4. It contains a eukaryotic origin of replication from an animal virus such as Simian virus 40 (SV40) and a prokaryotic origin of replication (from E. coli). The mammalian vector has a multiple cloning site and a selectable marker gene. Both of them are under the control of eukaryotic promoter and polyadenylation sequences.
Mammalian Expression Vector
These sequences are obtained from either animal viruses (SV40, herpes simplex virus) or mammalian genes (growth hormone, metallothionein). The promoter sequences facilitate the transcription of cloned genes (at the multiple cloning site) and the selectable marker genes. On the other hand, the polyadenylation sequences terminate the transcription. Ampicillin resistant marker gene can be used for selecting the transformed E. coli cells.
You didnt "write" any of it, you copied and pasted large chunks of text from someone else and are trying to say that you wrote it. Hell, you even copied parts that reference diagrams that you didn't bother to include. Keeping in mind, this isn't all the text you plagiarized, just a sampling of it. You really consider this "borrowing a few lines"?