How to Make Potato Dextrose Agar (PDA)

in mycelium •  7 years ago  (edited)
  1. To prepare potato infusion, boil 200 g sliced, unpeeled potatoes in 1 liter distilled water for 30 min.
  2. Filter through dishcloth, saving effluent, which is potato infusion.
  3. Mix with Dextrose (15g), Agar (15g) and Potato infusion and boil to dissolve.
  4. Autoclave and boil 15 min at 121°C, or use pressure cooker with 1.10 bar and boil 60 min.
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