[PhysioEX Chapter 11 exercise 3] PEX-11-03

Solved by ramonistry

Exercise 11: Blood Analysis: Activity 3: Hemoglobin Determination Lab Report

Pre-lab Quiz Results
You scored 100% by answering 3 out of 3 questions correctly.

1. A protein found in red blood cells, __________, is necessary for the transport of oxygen from the lungs to the cells of the
   body.
   You correctly answered: a. hemoglobin (Hb)
2. Anemia results when
   You correctly answered: e. Both b and c are correct.
3. How much hemoglobin does normal adult human blood contain?
   You correctly answered: c. 12-18 g/100 ml

Experiment Results

Predict Question:
Predict Question: How will the hemoglobin levels for the female Olympic athlete (sample 5) compare with the hemoglobin
levels for the healthy female (sample 2)?
Your answer : c. The hemoglobin levels for the female Olympic athlete will be greater than those for the healthy female.

Stop & Think Questions:
Why is the average hematocrit higher in males than in females?
You correctly answered: c. Higher testosterone levels in males promotes more RBC production.

Experiment Data:
Blood sample gm Hb per 100 ml blood Hematocrit (PCV) Ratio of PCV to Hb
1 16 48 3:1
2 14 44 3.14:1
3 8 40 5:1
4 20 60 3:1
5 22 60 2.73:1

Post-lab Quiz Results
You scored 100% by answering 3 out of 3 questions correctly.

1. To determine the amount of hemoglobin in a blood sample
   You correctly answered: e. All of these answers are correct.
2. Which of the following diseases is known to increase hemoglobin levels in an individual?
   You correctly answered: c. polycythemia.
3. Which of the following is known to decrease hemoglobin levels in an individual?
   You correctly answered: a. cirrhosis of the liver

Review Sheet Results

1. Is the male with polycythemia (sample 4) deficient in hemoglobin? Why?
   Your answer:
   No, cause polycythema means increasing of the number of erythrocytes.
2. How did the hemoglobin levels for the female Olympic athlete (sample 5) compare with the hemoglobin levels for the
   healthy female (sample 2)? Is either person deficient in hemoglobin? How well did the results compare with your
   prediction?
   Your answer:
   Hemoglobin level of the female Olympic athlete is higher than that of the healthy female, as predicted. Nobody is deficident
   in hemoglobin.
3. List conditions in which hemoglobin levels would be expected to decrease. Provide reasons for the change when
   possible.
   Your answer:
   Anemia, Cirrhosis, Hyperthyroidism, Renal disease, Hemorrhage.
4. List conditions in which hemoglobin levels would be expected to increase. Provide reasons for the change when
   possible.
   Your answer:
   Polycythermia, Heart failure, Obstructive pulmonary disease,
5. Describe the ratio of hematocrit to hemoglobin for the healthy male (sample 1) and female (sample 2). (A normal ratio of
   hematocrit to grams of hemoglobin is approximately 3:1.) Discuss any differences between the two individuals.
   Your answer:
   Ratio of male is 3:1. Ratio of female is 3.14:1.
   Maybe hormones and body mass make different ratio.
6. Describe the ratio of hematocrit to hemoglobin for the female with iron-deficiency anemia (sample 3) and the female
   Olympic athlete (sample 5). (A normal ratio of hematocrit to grams of hemoglobin is approximately 3:1.) Discuss any
   differences between the two individuals.
   Your answer:
   Ratio of female with iron-deficiency is 5:1. Ratio of female athlete is 2.73:1.
   Female with iron-deficiency has less hemoglobin and athlete has more erythrocytes.