[PhysioEX Chapter 11 exercise 4] PEX-11-04

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Exercise 11: Blood Analysis: Activity 4: Blood Typing Lab Report
Pre-lab Quiz Results
You scored 100% by answering 4 out of 4 questions correctly.

1. Red blood cell membranes have
   You correctly answered: c. agglutinogens that specify that individual's blood type.

2. ABO and Rh antigens
   You correctly answered: d. All of these answers are correct.

3. Which of the following statements about blood types is correct?
   You correctly answered: d. A person with type O blood has two recessive alleles and has neither the type A nor type B
   antigen.

4. Antibodies to the A and B antigens are
   You correctly answered: a. found preformed in the blood plasma.

Experiment Results

Predict Question:
Predict Question: If the patient's blood type is AB- , what would be the appearance of the A, B, and Rh samples?
Your answer : b. A, clumped; B, clumped; Rh, unclumped

Stop & Think Questions:
Why are individuals with the AB+ blood type known as universal recipients for blood transfusions?
You correctly answered: c. They have both A and B antigens on the surface of their RBCs, and their blood serum does not
contain antibodies against A, B, or Rh antigens.

Experiment Data:
Blood sample Agglutination with
Anti-A Serum
Agglutination with
Anti-B Serum
Agglutination with
Anti-Rh Serum
Blood Type
1 positive negative positive A+
2 negative positive positive B+
3 positive positive negative AB-
4 negative negative negative O-
5 positive positive positive AB+
6 negative positive negative B

Post-lab Quiz Results
You scored 100% by answering 4 out of 4 questions correctly.

1. To determine an individual's blood type, drops of the blood sample are mixed
   You correctly answered: a. separately with antiserum containing antibodies that recognize either type A antigens, type B
   antigens, or Rh antigens.

2. Agglutination
   You correctly answered: d. All of these answers are correct.

3. Antigens
   You correctly answered: b. are present on all cells.

4. ABO and Rh agglutinogens
   You correctly answered: c. cause the most vigorous and potentially fatal transfusion reactions.

Review Sheet Results

1. How did the appearance of the A, B, and Rh samples for the patient with AB- blood type compare with your prediction?
   Your answer:
   As my prediction, A clumped, B clumped and Rh clumped.

2. Which blood sample contained the rarest blood type?
   Your answer:
   Sample 3 with AB-.

3. Which blood sample contained the universal donor?
   Your answer:
   Sample 4 with O-.

4. Which blood sample contained the universal recipient?
   Your answer:
   Sample 5 with AB+.

5. Which blood sample did not agglutinate with any of the antibodies tested? Why?
   Your answer:
   Sample 4 with O-, because erythrocytes of the O- do not have any antigen A, B nor Rh.

6. What antibodies would be found in the plasma of blood sample 1?
   Your answer:
   Anti-B antibody.

7. When transfusing an individual with blood that is compatible but not the same type, it is important to separate packed
   cells from the plasma and administer only the packed cells. Why do you think this is done? (Hint: think about what is in
   plasma versus what is on RBCs.)
   Your answer:
   When people need blood, the erythrocytes matter. And in the plasma, it contains antibody which makes blood coagulation.

8. List the blood samples in this activity that represent people who could donate blood to a person with type B+ blood.
   Your answer:
   Sample 2, cause of same type with B+.
   Sample 4, cause of universal donor.
   Sample 6, cause - can donate to +.