amino
7 years ago by mcw (63)
$19.34
- Past Payouts $19.34
- - Author $15.94
- - Curators $3.40
176 votes
- + ripperone: $3.621 (14%)
- + hendrikdegrote: $2.794 (0.75%)
- + nicolemoker: $0.975 (50%)
- + guyverckw: $0.829 (60%)
- + wilkinshui: $0.735 (100%)
- + steemstem: $0.721 (5%)
- + htliao: $0.713 (25%)
- + thomaskikansha: $0.703 (100%)
- + kitcat: $0.622 (100%)
- + victorier: $0.585 (100%)
- + krischy: $0.443 (100%)
- + abigail-dantes: $0.406 (5%)
- + linuslee0216: $0.363 (100%)
- + goodboyphilip: $0.338 (100%)
- + justtryme90: $0.309 (2%)
- + kenchung: $0.285 (100%)
- + aaronli: $0.270 (100%)
- + carinewhy: $0.267 (100%)
- + curie: $0.239 (0.75%)
- + susanlo: $0.204 (80%)
- … and 156 more
Being A SteemStem Member
Downvoting a post can decrease pending rewards and make it less visible. Common reasons:
Submit
very interesting post friends, friends if there is time please visit my blog.
Downvoting a post can decrease pending rewards and make it less visible. Common reasons:
Submit
Thanks ~
Downvoting a post can decrease pending rewards and make it less visible. Common reasons:
Submit
Yeah friends do not forget ya!
Downvoting a post can decrease pending rewards and make it less visible. Common reasons:
Submit
Be careful at the time of using amino acid.
Downvoting a post can decrease pending rewards and make it less visible. Common reasons:
Submit
What do you mean "be careful"?
Downvoting a post can decrease pending rewards and make it less visible. Common reasons:
Submit
我终于知道臭屁是什么了。。。哈哈哈
Downvoting a post can decrease pending rewards and make it less visible. Common reasons:
Submit
🤣🤣🤣
Downvoting a post can decrease pending rewards and make it less visible. Common reasons:
Submit
好尴尬哈哈，下一期活动主题是什么呢，我要密切关注哈哈
Downvoting a post can decrease pending rewards and make it less visible. Common reasons:
Submit
哈哈這個可能要延一點
最近在趕進度沒有太多時間審閱文章🙈🙈
Downvoting a post can decrease pending rewards and make it less visible. Common reasons:
Submit
嗯我怕错过一天来看好几遍哈哈哈，因为我每次都要想好几天才能想起来适合的东西哈哈。看来我最近可以放松一下了哈哈
Downvoting a post can decrease pending rewards and make it less visible. Common reasons:
Submit
在出比賽的時候我在這裏留個言提醒一下吧 XDD
(如果我沒有忘記XD）
感謝支持啊！！！
Downvoting a post can decrease pending rewards and make it less visible. Common reasons:
Submit
Sometimes I really admire how you freely use those biological/chemical terms just like breathing. Great information tho!
Downvoting a post can decrease pending rewards and make it less visible. Common reasons:
Submit
🤣🤣🤣thanks!!!
Will that be too difficult to be understood?
Downvoting a post can decrease pending rewards and make it less visible. Common reasons:
Submit
I can tell you actually try hard to present them in the way eaiser to ubderstand, and you did it.
Downvoting a post can decrease pending rewards and make it less visible. Common reasons:
Submit
Thanks for the encouragement!!!
Downvoting a post can decrease pending rewards and make it less visible. Common reasons:
Submit
You'd think in micro/molecular bio, we'd be interested in L-cysteine as an amino acid (and we are, when we think of disulfide bridges).
You'd probably be suprised that on a day-to-day basis, we generally think of it as 'that reducing agent we add to anaerobic media to scrub out trace oxygen'. Unsurprisingly, a common amino acid tends to be rather non-toxic to the bugs we want to grow.
Downvoting a post can decrease pending rewards and make it less visible. Common reasons:
Submit
I didn't know that people are actually using cysteine to remove trace oxygen ?!
Well, lots of broth are just some peptides and amino acids, so cysteine should be there and should be non toxic (but also help bugs grow too ?!!) Haha XDDIs it really a general protocol? I thought it is just commonly used for scavenging ROS in cell.
Downvoting a post can decrease pending rewards and make it less visible. Common reasons:
Submit
Oh, absolutely. It's fairly common since it's so gentle and non-toxic.
The biggest problem is that it's also slow. We usually add resazuring as a redox indicator at the same time, prior to inoculation. We have to wait for it to go from pink to clear, and this can take a few hours at 37 C, even if we prepare using water boiled under N2/CO2 and keep an anoxic headspace and it's just scavenging the last traces of oxygen.
Downvoting a post can decrease pending rewards and make it less visible. Common reasons:
Submit
Forgot to add, we generally schedule anaerobic media prep around dinner/pub time for that reason.
Downvoting a post can decrease pending rewards and make it less visible. Common reasons:
Submit