Liquid Chromatography ( HPLC) Daily Routine maintenance

in agilent •  7 years ago  (edited)

Before daily analysis everyone has to check Liquid chromatography for better performance and also avoid the problem

1. Check mobile phase level.

    Mobile phase level play a vital role for running a LCMS (Liquid chromatography–mass spectrometry).Empty mobile phase creates air bubble in flow path thous pressure fluctuation and many problems.It is better to check mobile phase level first if level is low fill up.

2. Mechanical pump oil level and oil color checking. 

Oil level in rough pump of mass spectrometry should not be empty or lower than warning level.Color of oil should be colorless or light yellow color.Dark yellow or close to orange color of oil means , it should be changed.

3. Check nitrogen generator flow level, outlet level and operation pressure

Without proper nitrogen gas pressure, mass spectrometry  will be unstable vacuum condition.It will create problem with analysis.Checking nitrogen generator flow level and pressure always a wise decision.

4.Check column pressure

Column pressure should not exceed its operation condition.It will hamper column life time.Log should be maintained for pressure record.So that it can be easily notify that column pressure is higher than the usual.

5.Check solvent waste, dispose when full

   Solvent waste should check because of overflow of waste from disposal chemical bin.

6.Surface cleanup

Dust or other unwanted waste can create lot problem for analysis and also for flow path in capillary.Surface cleanup mandatory action for daily maintenance.


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nice post....👌

Thanks bro.