The invention of the ADA Assay Kit was the turning point in the field of bio-medical imaging. It is a non-imaging, nondestructive testing method that can be used for clinical diagnosis of various pathologies. The kit includes a probe, a labeling medium, a bead mix, a digestion solution, a micro plate and an exogenous DNA marker. Adenosine Deaminase (ADA) is a lymphocyte marker for T-cell proliferation. In BioVision's ADA Activity Assay, a mixture of inosine derived from the breakdown of adenosine in aqueous humor is activated by an enzyme-based fluorescent assay.
The probe included in the ADA activity assay is an exogenous DNA marker that is complementary to the probe of interest. The nucleoside phosphorylase (PN) gene is complementary to a fluorescently labeled dNTP. The NTP molecules are attached at specific sequence(s) within the nucleoside phosphorylase domain. Using arginine-based primer, the probe can be detected using an automated assay.
The ADA kit includes an easy-to-use manual and an instructional video. The manual is designed to explain the procedure in detail. The video shows images of gel stained cells taken from blood samples, which are representative of human adenosine deaminase (ADO) activity. The colorimetric results of the gel-staining are accurate and reliable. Both the manual and video guides include information on how to interpret the results and interpret the assay results for either titer or stain-time profile analysis. When enzyme bilirubin separate occurs in patients with severe hepatitis, ADA is increased significantly. Kangte Adenosine Deaminase Assay Kit.
The manufacturer recommends this product for use in the laboratory situation and not for diagnostic purposes. A test for predicting the activity of adenosine in the blood demonstrates a very low level of activity in normal controls. This is because the assay for detecting adenosine in the plasma is non-specific. There are no experimental requirements for using the serum ada activity kit in clinical settings.
To detect the activity of deaminase in human plasma and saliva, a sample of the subjects' blood is first collected. Samples are then analyzed according to the specifications set forth in the manufacture's manual. The manufacturers recommend that samples be processed immediately after collection. Because deaminase in the human blood sample may not degrade to the levels required for pharmaceuticals, biopsies and testing are often performed on animals before processing human samples.
The diazyme substrate mixture used in the testing procedure is made up of different concentrations of deaminase, glucose, and pH stabilizers. The pH stabilizers prevent the reaction between deaminase and substrate, thus preventing the production of high activity ADDA. In normal circumstances, the activity of deaminase and substrate is balanced by small amounts of pH buffer. When the pH of a culture plate is adjusted so that the concentration of deaminase in the medium is equal to the concentration of substrate in the culture plate, substrates are left in the incubation tank and incubated for up to two days. Substrates are then tested for the presence of detectable amounts of adenosine.
The kit includes a separate window for measuring pH, a test for substrate concentration, and test for alkalinity/alkalinity. The separate window for pH is designed so that pH results can be recorded during specific test procedures and pH results can be compared with test results from other plates. The test for alkalinity/alkalinity measures the pH of the samples in separate tubes that have been mixed with tap water. Adenosine molecules are detected using a methodology based on the monosodium glutamate (MSG) mechanism. The test for substrate concentration measures the amount of nonsubstances in the culture medium. All tubing, probes, and supporting solutions are FDA approved for use with human clinical trials.
The test for using tubes containing human urine as substrate showed that there is a high level of affinity between the acid and the non-acid pH of the human urine. The pigs' ability to form mixtures with different acid pH levels is hampered when this method is used because it requires diluting the samples before use. The test for using tubes containing human saliva as substrate showed that there is a high level of nonsubstances in the samples.