Understand Blast Scoring (Pair Alignment) | In Bangla

Pair-wise alignment is the key for BLAST search:

Pair-wise alignment score relies on 3 factors:

1. Positive hits/matches
2. Mismatches scores
3. Gap penalties

Identity score = Number of positive hits × score of each positive hit- {(Number of mismatches × Score of each mismatches) + (Number of gaps × score for each gap penalty)}