Today I sterilized some tomato seeds in preparation for in vitro germination.
To sterilize I soaked the seed for 15 minutes in an 8.5% Sodium Hypochlorite(clorox) solution. After soaking the seed it rinsed them 3 times with autoclaved deionized water.
I then placed the seeds in a standard Murashige and Skoog media(Sucrose, gellan gum, biocide, MS salt mix)
Seeds left on the Shelf to Germinate. I will be watching for signs of contamination each day.
Here is the live action...
Great article, very interesting. Thank you
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For anyone interested I will be posting the process of making the Murashige and Skoog media soon!
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very useful article @iamblake ... i thinkthe farmer must watch this video and read the article
thanks for sharing
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