@suesa I know this is an older post but I still am curious about how you are knocking out the brain specific transcript. I have made a figure of my understanding form your description. But my question is by inserting the Gene X could this not affect the normal splicing of the oocyte specific message?
RE: Let's Splice! (My Research, Vol. 3)
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Let's Splice! (My Research, Vol. 3)
Let's say it like this: we hope it doesn't influence the splicing of the oocyte specific version. The untranslated region we're adding the gene to is only found in the brain specific mRNA. If you're curious how it'll turn out, message me July 3rd as that's one day after I've turned in my thesis.
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Ok thanks for the clarification and good luck with your thesis write up and defence!
Here is a practice defence question for you:
Why don't you just delete the intron between the oocyte specific exon and exon-1 ( to make a combined larger exon) to "knock out" the brain specific transcript?
btw what is the proper protocol or etiquette for messaging users? I just discovered Discordapp.com and should I contact users there or in the comments section of their posts?
Cheers!
ian
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I think discord is generally better. And the deletion of the intron might lead to unforeseen problems with the oocyte specific transcript. But I don't really need to defend my bachelor thesis, as the topic is not picked by me but chosen by my supervisor :P
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