Damnn thats some pretty sexy science right there, I might've missed it but what gene were you looking for the mice to develop?? Would I be right to guess the plan that the successfully transgenic mice will also glow green?
RE: Microinjections - The Creation of Transgenic Mice (My Research, Vol. 6)
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Microinjections - The Creation of Transgenic Mice (My Research, Vol. 6)
They won't glow (it's actually fluorescence) green, as the GFP is only used as a marker very early on. And the inserted gene in question is actually not that important in my case, the method how it's inserted is the interesting thing. Trying to combine CRISPR/Cas9 with cre/loxp.
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